Flow Cytometry
Specification of Flow Cytometry
Optical System:
1. Laser configuration: 488nm solid-state laser is used, which can excite at least four fluorescent dyes at the same time, laser power ≥40mW;
Laser source | Fluorescence Channel | Recommended Fluorescent Fuel |
488nm | FL1:530/30nm | FITC |
FL2:585/40nm | PE、PI | |
FL3:670LP | PE-Cy5.5、PC5、PC5.5、PerCP、PerCP-Cy5.5 | |
FL4:785/60nm | PE-Cy7、PC7 |
2. Spatial three-dimensional excitation mode, fixed optical path without adjustment, overall temperature control of the optical system;
3. Linear optical path design, optical filters can be replaced with experimental needs;
4. The instrument is equipped with an avalanche photodiode (APD) to improve photoelectric conversion efficiency;
Analytical Performance:
1. Fluorescence sensitivity: FITC≤200MESF, PE≤100MESF;
2. Fluorescence Linearity:≥0.98;
3. Instrument detection resolution (full peak width CV of forward angle scattered light and fluorescence channel): FSC≤2.0%; FL1(FITC) ≤2.0%; FL2(PE)≤2.0%;
4. Resolution of forward angle scattered light and lateral angle scattered light: able to separate red blood cells and platelets in peripheral blood; able to separate the three groups of white blood cells (lymphocytes, monocytes and granulocytes) in peripheral blood;
5. Detection sensitivity of forward angle scattered light : ≤1.0μm;
6. Test carrying contamination rate ≤0.5%, after each test is completed, the flow chamber sample needle can be automatically cleaned to avoid cross contamination
7. Highest event rate : ≤50000 cells per second;
8. Compatible volume method and microsphere method absolute counting;
Sample Loading System:
1. Use a syringe to load the sample;
2. Compatible with a variety of centrifuge tubes and flow cytometry tubes;
3. With four different speed modes for sample loading, different modes can be selected according to actual sample processing requirements;
Sample speed adjustment
High Speed | Medium Speed | Low Speed | Sheath Speed |
124uL/min | 82uL/min | 33uL/min | 12mL/min |
Software System:
1. Full-matrix fluorescence compensation,the instrument supports online and offline compensation;
2. The single software supports data acquisition and data analysis at the same time and can edit reports on the software, and can directly generate FCS files without special operating software;
3. The operating system supports Microsoft WIN10 system;
Others:
The host can be placed in a single clean bench for test operations
Product Physical Characteristics :
Dimension:250mm*280mm*250mm
Weight:10KG
Power Supply :AC 100-240V,50/60Hz